Soyabean Casein Digest Agar (Tryptone Soya Agar)

Soyabean Casein Digest Agar – Tryptone Soya Agar

Code: M290-500g

Soyabean Casein Digest Agar (Tryptone Soya Agar): is a general purpose medium used for cultivation of a wide variety of microorganisms and for sterility testing in pharmaceutical procedures. It is the Microbiology Culture Media of Himedia Laboratories Pvt.

Directions of Soyabean Casein Digest Agar – Tryptone Soya Agar: 

Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, aseptically add 5% v/v defibrinated blood in previously cooled medium to 45-50°C for cultivation. Mix well and pour into sterile Petri plates.

Quality Control of Soyabean Casein Digest Agar – Tryptone Soya Agar:

Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Basal Medium : Light yellow coloured clear to slightly opalescent gel. After addition of 5-7%w/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates pH of 4.0% w/v aqueous solution at 25°C .
pH: 7.10-7.50
Cultural response: Cultural characteristics was observed after an incubation for Bacterial at 30-35°C 18-24 hours and for Fungal at 30-35°C <=5days

Storage and Shelf Life:

Store below 30°C in tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.