Lauryl Sulphate Broth

Lauryl Sulphate Broth

Code: M080 -500g

Lauryl Sulphate Broth (Lauryl Tryptose Broth): is used for the detection of coliforms in water, wastewater, dairy products and other food samples. It is the Microbiology Culture Media of Himedia Laboratories Pvt.

Directions of Lauryl Sulphate Broth: 

Suspend 35.6 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes containing inverted Durhams tubes.

Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For inoculum of 1 ml or less, use single strength medium. For inocula of 10 ml or more, double strength or proportionate medium should be prepared.

Quality Control of Lauryl Sulphate Broth: 

Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured, clear solution without any precipitate
Reaction: Reaction of 3.56% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH: 6.60-7.00
Cultural Response: M080: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Storage and Shelf Life:

Store below 30°C in tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.