Xylose-Lysine Deoxycholate Agar

Xylose-Lysine Deoxycholate Agar

Code: M031 – 500g

Xylose-Lysine Deoxycholate Agar (XLD Agar) is a selective medium recommended for the isolation and enumeration of Salmonella Typhi and other Salmonella species. It is the Microbiology Culture Media of Himedia Laboratories Pvt.

Directions of Xylose-Lysine Deoxycholate Agar: 

Suspend 56.68 grams in 1000 ml distilled water. Heat with frequent agitation until the medium boils. DO NOT AUTOCLAVE OR OVERHEAT. Transfer immediately to a water bath at 50°C. After cooling, pour into sterile Petri plates. It is advisable not to prepare large volumes that will require prolonged heating, thereby producing precipitate.
Note : Slight precipitation in the medium may occur,which is inheritant property of the medium,and does not affect the performance of the medium.

Quality Control of Xylose-Lysine Deoxycholate Agar:

Appearance: Light yellow to pink homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Red coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 5.67% w/v aqueous solution at 25°C . pH : 7.4±0.2
pH: 7.20-7.60
Cultural Response: Cultural response was observed after an incubation at 35-37°C for specified time. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.

Storage and Shelf Life:

Store below 30°C in a tightly closed container and use freshly prepared medium. Use before expiry date on the label.