Mannitol Salt Agar

Mannitol Salt Agar

Code: M118-500g

Mannitol Salt Agar is used as a selective media for the isolation of pathogenic Staphylococci and It is the Microbiology Culture Media of Himedia Laboratories Pvt.

Directions of Mannitol Salt Agar:

Suspend 111.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes.Cool to 45-50°C. If desired, add 5% v/v Egg Yolk Emulsion (FD045). Mix well
and pour into sterile Petri plates or dipense as desired.
Note : This product contains 7.5% Sodium chloride as one of its ingredients. On repeated exposure to air and absorption
moisture sodium chloride has tendency to form lumps, therefore we strongly recommend storage in tightly closed containers
in dry place away from bright light

Quality Control of Mannitol Salt Agar:

Appearance: Light yellow to pink homogeneous free flowing powder
Gelling: Firm,comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Red coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 11.1% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH: 7.20-7.60
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar

Storage and Shelf Life:

Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label